A stable LifeAct-TagGFP2 expressing human fibrosarcoma cell line
Long-term actin visualization - 25 passages guaranteed
Characteristics proven to be identical to the parental HT-1080 cell line
LifeAct technology guarantees no interference with cytoskeletal dynamics
Applications:
Live cell imaging of cytoskeletal organization and dynamics
Functional cell-based assays, such as chemotaxis and migration
Usable as a characterized control in your experiments
To create the stable LifeAct-TagGFP2 expressing cell line, human fibrosarcoma cells were stably transfected with a plasmid coding for TagGFP2-labeled LifeAct .
The HT-1080 LifeAct-TagGFP2 cells present highly dynamic filamentous actin. As a result of stable LifeAct expression, perfect visualization of filamentous actin with very low background fluorescence is achieved without any interference to cellular dynamics. Moreover, full characterization of the cells revealed that they displayed identical behavior to wild type (wt) cells.
Due to the cell line’s fast growth and the absence of contact inhibition, it constitutes an ideal tumor model. These characteristics combined with bright and specific F-actin staining lead to a powerful tool with versatile applications in live cell imaging and high-content approaches.
Specifications:
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Cells/vial
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5 x 105
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Medium
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ibidi Freezing Medium
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Storage
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Liquid nitrogen
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Biosafety Level
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1
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Sterility
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Tested for yeast and bacteria
including mycoplasma
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Promoter
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CAG (modified chicken β-actin)
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Transgene
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LifeAct-TagGFP2*
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*For further information on TagGFP2, please refer to www.evrogen.com.
Origin information:
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Organism
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Homo sapiens (human)
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Source
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Connective tissue disease: fibrosarcoma
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Age
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35 years
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Gender
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Male
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Ethnicity
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Caucasian
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Growth properties
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Adherent
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Morphology
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Epithelial
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Technical Features:
Homogeneous cell pool
Assured cell line identity
Purity tested
Optimal transgene expression for microscopy assays