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Excellent Phase Contrast in the μ-Slide 4 well Ph+
An open μ-Slide with 4 wells and a special intermediate plate for excellent phase contrast and high-end fluorescence microscopy
Excellent phase contrast over the entire well – no meniscus
All-in-one chamber used for cell culture and microscopy
Observation of the sample through the coverslip-like bottom using high resolution microscopy
Fast and easy immunofluorescence, with no coverslips and no leakage
Applications:
Cultivation and microscopy of cell cultures
Transfection assays
Fluorescence microscopy of both living and fixed cells
Live cell imaging over extended time periods
Specifications:
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Number of wells
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4
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Dimensions of wells
(w x l x h) in mm
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21.2 x 11.0 x 3.0
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Volume per well
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700 μl
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Total height with lid
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10.8 mm
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Growth area per well
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2.2 cm2
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Coating area per well
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5.9 cm2
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Bottom:
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ibidi Standard Bottom
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Technical Features:
Open μ-Slide with 4 independent wells
Intermediate plate in each well to avoid meniscus formation
Easy filling, without air bubbles, through the lateral slot
Excellent optical quality for high-end microscopy
Compatible with staining and fixation
ibiTreat surface for optimal cell adhesion
Made of biocompatible plastic material – no glue and no leaking
Phase contrast microscopy is the most commonly used, transmitted light technique in cell culture. When working with phase contrast microscopy, it is crucial to have the two phase rings adjusted to each other. In open wells, the meniscus at the air-water-interphase works like a lens that refracts the beam path. This miscalibrates the phase rings, leading to poor contrast in the microscopic image.
μ-Slide 4 well μ-Slide 4 well Ph+
 
 
Working with the μ-Slide 4 well Ph+ diminishes the meniscus, so that the whole optical system is aligned, no matter which position of the well is being imaged.
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Cross Section Through a Well of the μ-Slide 4 well Ph+ with a Transmitted Light Beam Path
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The illustration on the left shows the perturbing effect of a meniscus. Light is refracted on the air-water-interface, leading to poor contrast in microscopy. Only the small center part exhibits satisfying phase contrast.
Working with the μ-Slide 4 well Ph+ diminishes the meniscus and increases the area of nicely contrasted cells. This nice contrast is due to the parallel beam path that is created by the plate.
Comparison Ph+ Well versus Standard Well:
No Meniscus Effect in Ph+ Well
μ-Slide 4 well Ph+
Excellent Phase Contrast
Excellent Fluorescence Microscopy
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Standard Well
Poor Phase Contrast
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